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SRP0147 Sigma

PRMT6 human

recombinant, expressed in FreeStyle 293-F cells, ≥70% (SDS-PAGE)

Synonym: Arginine methyltransferase 6, HMT1 hnRNP methyltransferase-like 6, HRMT1L6

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Properties

Related Categories Epigenetics, Histone Modifying Enzymes, Methyltransferases, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in FreeStyle 293-F cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 35 kDa
NCBI accession no.   NM_018137
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PRMT6(55170)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human PRMT6 (GenBank Accession No. NM_018137), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 35 kDa, expressed in FreeStyle 293-F cells.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 100 μg/ml FLAG peptide, 10% glycerol and 3 mM DTT.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

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