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SRP0161 Sigma

Histone H4 peptide (1-21) human

≥90% (HPLC)

Synonym: HIST2H4A

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Properties

Related Categories Cell Culture, Epigenetics, Growth Factors and Cytokines, Histones, Molecular Biology,
assay   ≥90% (HPLC)
form   aqueous solution
mol wt   mol wt ~2 kDa
NCBI accession no.   NM_003548
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... HIST2H4A(8370)

Description

Application

Study enzyme kinetics, and screen small molecular inhibitors of Histone 3 R3 methyltransferases for drug discovery and HTS applications

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube to recover full content of the tube. Aliquot into single use aliquots. Store remaining product in aliquots at -70°C. Note: Avoid freeze/thaw cycles.

Physical form

Supplied as water solution, 800 μM

Biochem/physiol Actions

H4 (histone 4) is a core histone protein which is mobilized during herpes simplex virus 1 (HSV1) infection. This leads to elevated pools of free H2B and H4 and decreased rate of H2B fast chromatin exchange. Free H4 associates with HSV1 genomes, and this mobilization is also linked with the organization of H2A-H2B and H3-H4 dimers into unstable nucleosomes with HSV-1 genomes. The histone chaperone nuclear autoantigenic sperm protein (NASP) is responsible for maintaining a reservoir of soluble H3-H4 histones, where it regulates the activity of heat shock proteins Hsc70 and Hsp90, which target H3-H4 for degradation by chaperone-mediated autophagy. This reservoir level, in turn, depends upon the level of NASP.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Peer-Reviewed Papers
15

References

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