• USA Home
  • SRP0162 - JMJD3/KDM6B Active human

EMAIL THIS PAGE TO A FRIEND
SRP0162 Sigma

JMJD3/KDM6B Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym: JmjC domain-containing protein 3, KDM6B, KIAA0346, lysine (K)-specific demethylase 6B

Purchase

Properties

Related Categories Demethylases, Epigenetics, Histone Modifying Enzymes, Molecular Biology, Proteins and Peptides for Epigenetic Research More...
recombinant   expressed in baculovirus infected insect cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 71 kDa
NCBI accession no.   NM_001080424
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... KDM6B(23135)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 3 mM DTT.

Biochem/physiol Actions

KDM6B (lysine demethylase 6B) is responsible for the demethylation of histone H3K27. At promoters it demethylates the repressive H3K27me3 and therefore, promotes gene expression. In human and mouse fibroblasts, it demethyates the H3K27me3 at INK4a/ARF (alternate reading frame) locus, thus, promoting the expression of p16 protein, and therefore, acts as a tumor suppressor. It is also involved in inflammatory pathways in macrophages and embryonic stem cell differentiation. The expression of KDM6B is elevated in clear cell renal cell carcinoma (ccRCC) where it promotes EMT (epithelial to mesenchymal transition). Increased expression is also linked positively with lymph node metastasis, tumor size, cancer stage and poor prognosis.

General description

KDM6B (lysine demethylase 6B)/ JMJD3 (jumonji domain-containing 3) is a major histone demethylase protein. This protein contains a JmjC domain in its C-terminal.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?