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SRP0166 Sigma

DNMT3B/DNMT3L Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonym: DNA (cytosine-5-)-methyltransferase 3b, ICF, M.HsaIIIB

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Properties

Related Categories Cell Culture, DNMTs, Epigenetics, Growth Factors and Cytokines, Histone Modifying Enzymes,
recombinant   expressed in baculovirus infected insect cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 27 kDa (DNMT3L)
  mol wt 59 kDa (DNMT3B)
NCBI accession no.   NM_006892 (DNMT3B)
  NM_013369 (DNMT3L)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... DNMT3B(1789)

Description

Application

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCL, 20% glycerol, 4 mM glutathione.

Biochem/physiol Actions

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) is a de novo methyltransferase which targets the un-methylated 5′-C-phosphate-G-3′ (CpG) sites on the gene promoter. It is responsible for crucial epigenetic mechanisms for the regulation of chromosomal stability and for building a new methylation pattern. The protein is associated with promoter hypermethylation in tumorigenesis and lung cancer. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is a non-catalytic accessory factor which is mainly responsible for the stimulation of DNA methylation. It helps the DNA methylation machinery to be positioned on properly chromatinized DNA regions. The protein also helps to provide uniform methylation patterns. It has an important role in spermatogenesis and mutations in the DNMT3L gene might be associated with azoospermia susceptibility.

General description

DNA (cytosine-5-)-methyltransferase 3 β (DNMT3B) has two subfamilies-DNMT3B and ΔDNMT3B. These are expressed in many transcript variants. Several single-nucleotide polymorphisms are observed within the promoter region of the DNMT3B gene. The gene encoding this protein is localized on human chromosome 20q11.2. DNA (cytosine-5)-methyltransferase 3-like (DNMT3L) is part of the DNA methyltransferases family. It has a cysteine-rich region containing a novel-type zinc finger domain. The gene encoding it is localized on human chromosome 21q22.3.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

Peer-Reviewed Papers
15

References

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