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SRP0167 Sigma

DNMT3B Active from mouse

recombinant, expressed in baculovirus infected insect cells, ≥30% (SDS-PAGE)

Synonym: DNA (cytosine-5-)-methyltransferase 3b, DNA MTase MmuIIIB

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Properties

Related Categories Cell Culture, DNMTs, Epigenetics, Growth Factors and Cytokines, Histone Modifying Enzymes,
recombinant   expressed in baculovirus infected insect cells
assay   ≥30% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 99 kDa
NCBI accession no.   NM_001003961
shipped in   dry ice
storage temp.   −70°C
Gene Information   mouse ... DNMT3B(13436)

Description

Application

Useful for the study of DNA methylation, screening inhibitors, and selectivity profiling.

General description

Murine DNA methyltransferase 3B (DNMT3B) (GenBank Accession No. NM_001003961), amino acids 2-859 (end), with N-terminal His-tag and C-terminal FLAG-tag, MW = 99 kDa, expressed in Sf9 cells via a Baculovirus expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Price and Availability

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

Modulators and Proteins for Methylation

DNA methyltransferases are a family of enzymes that catalyze the transfer of a methyl group to DNA. There are five related DNA cytosine-5-methyltransferases (DNMTs) that transfer a methyl group from ...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 7–9
Keywords: Gene expression, Methylations, PAGE

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