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SRP0177 Sigma

Histone H3 full length human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym: HIST1H3E, Histone H3.1



Related Categories Cell Culture, Epigenetics, Growth Factors and Cytokines, Histones, Molecular Biology,
recombinant   expressed in E. coli
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 15.4 kDa
NCBI accession no.   NM_003532
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... HIST1H3E(8353)



Suitable substrate for histone methyltransferases

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing protein to recover full content of the tube. Aliquot protein into single use aliquots. Store remaining undiluted protein in aliquots at -70°C. Note: Protein is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 10 mM PBS, pH 7.4, 130 mM NaCl, 10% glycerol and 1 mM DTT.

Biochem/physiol Actions

Histones are important for proper organization and modification of chromatin. HIST1H3E (histone 3, also referred to as H3.1) is mainly associated with DNA synthesis during DNA replication and might also be involved in DNA repair. It is added to the chromatin at the time of DNA replication-associated chromatin assembly. During HSV-1 (herpes simplex virus 1) infection, differential movement of HIST1H3E is associated with the assembly of viral chromatin.

General description

The gene HIST1H3E (histone 3, also referred to as H3.1) is mapped to human chromosome 6p22. It is a variant of histone 3 and is also called as the replicative histone. HIST1H3E is mainly expressed in the S-phase. In the process of cellular differentiation, HIST1H3E mRNA levels goes down as cell division slows down.
Human HIST1H3E, GenBank Accession No. NM_003532, amino acids 2-137 (end) with N-terminal His-tag, MW = 15.4kDa, expressed in an Escherichia coli expression system.

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Histone Modification and Chromatin Remodeling

Gene expression is governed by complex mechanisms including transcription factor binding to DNA and coordinated changes in chromatin structure. The primary protein components of chromatin are the his...
Savita Bagga, PhD.
BioFiles v7 n3, 2012, 10–16
Keywords: Acetylations, Amplification, Cancer, Cell culture, Chromatin immunoprecipitation, Cloning, DNA purification, Diseases, Gene expression, Immunoprecipitation, Indicators, Methylations, Microarray Analysis, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Polymorphisms, Purification, Sequencing, Transcription, Whole genome amplification

Peer-Reviewed Papers


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