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SRP0179 Sigma

Lysyl Oxidase-like 2 (LOXL2) FLAG-tag (Sf9-derived) human

recombinant, expressed in baculovirus infected insect cells, ≥65% (SDS-PAGE)

Synonym: LOR2, LOXL2, WS9-14

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix, Extracellular Matrix, Extracellular Matrix Peptides and Proteins More...
recombinant   expressed in baculovirus infected insect cells
assay   ≥65% (SDS-PAGE)
form   aqueous solution
NCBI accession no.   NM_002318
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... LOXL2(4017)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT

Biochem/physiol Actions

LOXL2 (lysyl oxidase like 2) is an amino oxidase which is dependent on copper and quinone. LOXL2 participates in various events, such as gene transcription, cell migration, adhesion, angiogenesis and differentiation. It is responsible for connecting collagen and/or elastin in the extracellular matrix. It deaminates trimethylated lysine 4 in histone H3, causing transcriptional repression. LOXL2 also oxidises methylated TAF10 (TATA-box binding protein associated factor 10), suppressing some TFIID (transcription factor IID)-associated genes. The LOXL2 gene is upregulated in various cancers, such as gastric cancer, breast cancer and squamous cell carcinomas. The gene is associated with cancer cell invasion, lymph node metastasis and poor overall patient survival.

General description

The gene LOXL2 (lysyl oxidase like 2) is mapped to human chromosome 8p21.3. It is widely expressed and belongs to the LOX family of proteins. The protein has a conserved C-terminus region with catalytic function and a divergent N-terminal part.
Human LOXL2 (GenBank Accession No. NM_002318), amino acids 500-end, MW = 32kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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