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SRP0186 Sigma

Bcl-2 Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym: Apoptosis regulator Bcl-2, B-cell CLL/lymphoma 2, B-cell leukemia/lymphoma 2

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Properties

Related Categories Apoptosis and Cell Cycle, Bcl-2 Family Proteins, Bcl-2 Family Proteins and Antibodies, Cell Biology, Cell Signaling and Neuroscience More...
recombinant   expressed in E. coli
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 27 kDa
NCBI accession no.   P10415
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... BCL2(596)

Description

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Application

Useful for the study of apoptosis, screening inhibitors and selectivity profiling.

Biochem/physiol Actions

B-cell lymphoma 2 (BCL-2) inhibits apoptosis in cells and has been shown to be upregulated in adenomas.

General description

B-cell lymphoma 2 (BCL-2) is an anti-apoptotic and target gene of nuclear factor-κB (NF-κB). The protein is expressed in the nuclear envelope, mitochondria and endoplasmic reticulum. The gene encoding BCL-2 is localized on human chromosome 18q21. Human Bcl-2 (amino-acids 1-211) (GenBank Accession No. P10415) with C-terminal His tag, MW = 27 kDa, expressed in an E. coli expression system.

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Flow Cytometry Instruments

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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