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SRP0188 Sigma

Pyruvate kinase M2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: M2-PK tumor, OPA-interacting protein 3 (OIP-3), Pyruvate kinase 3 (PK3), Pyruvate kinase, muscle, Thyroid hormone-binding protein 1 (THBP1)



Related Categories Aerobic Glycolysis (the Warburg Effect), Cancer Metabolism, Cancer Research, Cell Biology, Cell Signaling and Neuroscience,
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 58.9 kDa
NCBI accession no.   NM_002654
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PKM2(5315)



Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem/physiol Actions

Pyruvate kinase M2 (PKM2) is an isoform of pyruvate kinase that is expressed in certain differentiated cells and in proliferatively active cells. PKM2 is often overexpressed in cancer cells.

General description

Human Pyruvate kinase M2 (PKM2) (GenBank Accession No. NM_002654) full length with N-terminal His tag, MW = 58.9 kDa, expressed in an E. coli expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to transfer a phosphoryl group from phosphoenolpyruvate (PEP) to 1 pmol of ADP/min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Aerobic Glycolysis and the Warburg Effect

The Warburg effect is the enhanced conversion of glucose to lactate observed in tumor cells, even in the presence of normal levels of oxygen. Otto Heinrich Warburg demonstrated in 1924 that cancer ce...
Keywords: Aerobic, Biofiles, Cancer, Cell proliferation, Clinical, Glycolysis, PAGE, Phosphorylations, Support, Transcription

Peer-Reviewed Papers


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