SRP0194 Sigma

PARP3 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: ADPRT3, ADPRTL2, IRT1, NAD(+) ADP-ribosyltransferase 3, Poly (ADP-ribose) Polymerase 3



Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Drug Discovery Enzymes, PARP (Poly(ADP-Ribose) polymerase),
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 87 kDa
NCBI accession no.   NM_001003931
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PARP3(10039)



Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human PARP3 (GenBank Accession No. NM_001003931), full length with N-terminal GST tag, MW = 87 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Price and Availability

Flow Cytometry Instruments

All labs need water

Amicon Ultra Centrifugal Filters
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles
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