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SRP0195 Sigma

PARP6 Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym: MGC131971, Poly (ADP-ribose) Polymerase 6, pART17

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Drug Discovery Enzymes, PARP (Poly(ADP-Ribose) polymerase),
recombinant   expressed in baculovirus infected insect cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 98 kDa
NCBI accession no.   NM_020214
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PARP6(56965)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute (room temperature) from NAD into acid-insoluble form.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Biochem/physiol Actions

PARP6 (poly(ADP-ribose) polymerase family member 6) negatively controls cell cycle, as its ectopic expression in HeLa cells leads to suppression of cell growth. It functions as a tumor suppressor in CRC (colorectal cancer), and its expression is associated with good prognosis. The ectopic expression of PARP6 results in suppressed expression of the oncogene survivin. In CRC PAPR6 prevents colony formation, invasion, migration, and cell proliferation.

General description

PARP6 (poly(ADP-ribose) polymerase family member 6) gene is localized to human chromosome 15q23. It is a member of the family of multifunctional nuclear proteases known as PARPs, which contains 17 members and is further classified into three subtypes dependent on the catalytic domain structure. PARP6 is a type of mono(ADP-ribosyl) transferase on the basis that its catalytic domain has a significantly shorter nicotinamide-ribose-binding site than PARP1. This subfamily contains nine members including PARP6. PARP6 is composed of 630 residues and has a molecular weight of 71kDa.
Human PARP6 (GenBank Accession No. NM_020214), full length with N-terminal GST tag, MW = 98kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Price and Availability


Flow Cytometry Instruments

All labs need water

Amicon Ultra Centrifugal Filters
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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