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SRP0198 Sigma

PARP11 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: C12orf6, DKFZp779H0122, MIB006, Poly (ADP-ribose) Polymerase 11

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Signaling and Neuroscience, Drug Discovery Enzymes, PARP (Poly(ADP-Ribose) polymerase),
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 65.6 kDa
NCBI accession no.   NM_020367
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PARP11(57097)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human PARP11 (GenBank Accession No. NM_020367), full length with N-terminal GST tag, MW = 65.6 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of PARP incorporates 100 pmoles of poly(ADP) in 1 minute from NAD into acid-insoluble form.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Price and Availability


Flow Cytometry Instruments

All labs need water

Amicon Ultra Centrifugal Filters
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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