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SRP0210 Sigma

PRL-3 (2-173) Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: PRL-3, PTP4A3, Protein tyrosine phosphatase type IVA, member 3, Protein-tyrosine phosphatase of regenerating liver 3

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Tyrosine Phosphatase (TP), Tyrosine Phosphatase Biology,
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 19.5 kDa
NCBI accession no.   NM_032611
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PTP4A3(11156)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human PRL 3 (GenBank Accession No. NM_032611), (amino acids 2-173) with N-terminal HIS tag, MW = 19.5 kDa, expressed in an E Coli expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 20% glycerol and 3 mM DTT.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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