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SRP0218 Sigma

TC-PTP Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: PTPN2, T-cell protein-tyrosine phosphatase, TC-PTP

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Tyrosine Phosphatase (TP), Tyrosine Phosphatase Biology,
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 62.3 kDa
NCBI accession no.   BC016727
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PTPN2(5771)

Description

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.

General description

Protein tyrosine phosphatase, Catalytic domain, (amino acids 2-315), (Gene bank accession number BC016727) with N-terminal GST tag, MW = 62.3 kDa, expressed in an E. coli expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
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