SRP0220 Sigma

DEP1 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym: CD148, DEP-1, Density-enhanced phosphatase 1, PTPRJ, Protein tyrosine phosphatase, receptor type, J



Related Categories Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Tyrosine Phosphatase (TP), Tyrosine Phosphatase Biology,
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 58.9 kDa
NCBI accession no.   NM_U10886
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PTPRJ(5795)



Useful for the study of enzyme kinetics, regulation, and to dephosphorylate target substrates.

General description

Protein tyrosine phosphatase (density-enhanced phosphatase-1, PTP-eta, CD148), Catalytic domain, (amino acids 1024-1306) (Gene bank accession no. NM_U10886) with N-terminal GST tag, MW=58.9 kDa, expressed in an E. coli expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Price and Availability

All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers


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