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SRP0225 Sigma

Rheb Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonym: GTP-binding protein Rheb, Ras homolog enriched in brain

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Properties

recombinant   expressed in baculovirus infected insect cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 23 kDa
NCBI accession no.   NM_005614
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... RHEB(6009)

Description

Application

Useful for the study of biochemical function.

General description

Human Rheb (GenBank Accession No. NM_005614), full length with N-terminal HIS6 tag, MW = 23 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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