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SRP0228 Sigma

SMURF2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: E3 ubiquitin ligase SMURF2, MGC138150, SMAD specific E3 ubiquitin protein ligase 2

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Intracellular Protein Degradation, Nitric Oxide and Cell Stress, Ubiquitin and Proteases More...
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 72 kDa
NCBI accession no.   NM_022739
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... SMURF2(64750)

Description

Application

Useful in conjunction with E1 and E2 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human SMURF 2 (GenBank Accession No. NM_022739), amino acids 130-end, with N-terminal DDDDK tag (FLAG), MW = 72 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to transfer 1 pmol of ubiquitin to substrate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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