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  • SRP0235 - PI3 kinase [p110a(H1047R)/p85a] Active human

SRP0235 Sigma

PI3 kinase [p110a(H1047R)/p85a] Active human

recombinant, expressed in baculovirus infected insect cells, ≥60% (SDS-PAGE)

Synonym: PIK3CA, Phosphoinositide-3-kinase, catalytic α, PtdIns-3-kinase p110



Related Categories Active Kinases, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Serine/Threonine Kinase Biology,
recombinant   expressed in baculovirus infected insect cells
assay   ≥60% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 129 kDa (p110α)
  mol wt 88 kDa (p85α)
NCBI accession no.   U79143 (p110alpha)
  XM_043865 (p85alpha)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PIK3CA(5290)



Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Complex of N-terminal FLAG-tagged recombinant full-length human p110α (GenBank Accession No. U79143), with H1047R mutation, and recombinant full length, human p85α (no tag, GenBank Accession No. XM_043865). Co-expressed in Sf9 cells in a Baculovirus expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

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