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SRP0236 Sigma

PI3 kinase (p110d/p85a) Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: PI3-kinase p110 subunit delta, PIK3CD, Phosphoinositide-3-kinase, catalytic, delta, ptdIns-3-kinase subunit p110-δ

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Properties

Related Categories Active Kinases, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Serine/Threonine Kinase Biology,
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 146 kDa (p110δ)
  mol wt 83.5 kDa (p85α)
NCBI accession no.   NM_005026 (p110delta)
  XM_043865 (p85alpha)
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PIK3CD(5293)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Complex of N-terminal GST tagged recombinant full-length human p110δ (GenBank Accession No. NM_005026), and recombinant full length, human p85α, GenBank Accession No. XM_043865). Coexpressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

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