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SRP0237 Sigma

PI3 kinase (p120g) Active human

recombinant, expressed in baculovirus infected insect cells, ≥90% (SDS-PAGE)

Synonym: PIK3CG, Phosphoinositide-3-kinase, catalytic, gamma, p120-PI3K

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Properties

Related Categories Active Kinases, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology, Serine/Threonine Kinase Biology,
recombinant   expressed in baculovirus infected insect cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 131 kDa
NCBI accession no.   AF327656
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PIK3CG(5294)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human p120γ ( GenBank Accession No. AF327656), full length with N-terminal His tag, MW = 131 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of kinase activity is defined as the amount of enzyme required to produce 1 pmol of Phosphoinositol-3, 4,5-Trisphosphate/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

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Safety Information

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Documents

Certificate of Analysis

Protocols & Articles
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