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SRP0240 Sigma

EGFR (L858R) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym: ERBB1, Epidermal growth factor receptor, HER1, PIG61, Proto-oncogene c-ErbB-1

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Properties

Related Categories Active Kinases, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors,
recombinant   expressed in baculovirus infected insect cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 88 kDa
NCBI accession no.   NM_005228
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... EGFR(1956)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human EGFR L858R (GenBank Accession No. NM_005228), (amino acids 668-1210) with N-terminal GST tag, MW = 88 kDa, expressed in baculovirus expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

EGFR Pathway

Background EGF Receptors & Ligands    Receptor Activation EGFR Signaling    Ras/Raf Signaling Cascade    Phosphatidylinositol 3-kinase/Akt Signaling Cascade    Signal Transducers and Activators of Tr...
Keywords: Apoptosis, Cancer, Cell proliferation, Central Nervous System, Clinical, Degradations, Diseases, Electron spin resonance, Gastrointestinal, Gene expression, Genetic, Glycosylations, Growth factors, Ligands, Morphogenesis, Transcription, Transduction

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