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SRP0242 Sigma

EGFR (T790M L858R) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym: ERBB1, Epidermal growth factor receptor, HER1, PIG61, Proto-oncogene c-ErbB-1

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Properties

Related Categories Active Kinases, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors,
recombinant   expressed in baculovirus infected insect cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 88 kDa
NCBI accession no.   NM_005228
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... EGFR(1956)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Biochem/physiol Actions

Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers.

General description

Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. EGFR can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor-α (TGF-α), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin and epigen.The gene encoding it is localized on human chromosome 7p11.2. Human EGFR T790M L858R (GenBank Accession No. NM_005228), (amino acids 668-1210) with N-terminal GST tag, MW = 88 kDa, expressed in baculovirus expression system.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles

Articles

EGFR Pathway

Background EGF Receptors & Ligands    Receptor Activation EGFR Signaling    Ras/Raf Signaling Cascade    Phosphatidylinositol 3-kinase/Akt Signaling Cascade    Signal Transducers and Activators of Tr...
Keywords: Apoptosis, Cancer, Cell proliferation, Central Nervous System, Clinical, Degradations, Diseases, Electron spin resonance, Gastrointestinal, Gene expression, Genetic, Glycosylations, Growth factors, Ligands, Morphogenesis, Transcription, Transduction

Peer-Reviewed Papers
15

References

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