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SRP0250 Sigma

PLK3 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: CNK, Cytokine inducible kinase, FGF-inducible kinase (FNK), Polo-like kinase 3, Proliferation-related kinase (PRK)

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Properties

Related Categories Active Kinases, Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Kinase/Phosphatase Biology,
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 36.7 kDa
NCBI accession no.   NM_004073
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PLK3(1263)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human Plk3 (GenBank Accession No. NM_004073), (amino acids 58-339) with N-terminal HIS6 tag, MW = 36.7 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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