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SRP0253 Sigma

4EBP1 Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym: EIF4EBP1, Eukaryotic translation initiation factor 4E binding protein 1, PHAS-I, Phosphorylated heat- and acid-stable protein regulated by insulin 1, eIF4E-binding protein 1

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Gene Regulation, Gene Regulation and Expression
recombinant   expressed in E. coli
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 13.6 kDa
NCBI accession no.   NM_004095
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... EIF4EBP1(1978)

Description

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 50% glycerol.

Application

4EBP1 Active human has been used as a substrate for in vitro kinase assay.

Biochem/physiol Actions

E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is involved in protein synthesis, cell growth and survival. In unphosphorylated form, it suppresses the translation initiation of capped mRNAs. On the other hand, in phosphorylated form, it increases translation initiation. Phosphorylation of E1F4EBP1 releases it from eIF4E (eukaryotic translation initiation factor 4E), thereby resulting in cap-dependent translation. E1F4EBP1 is also involved in tumorigenesis and the gene is overexpressed in various carcinomas. It is also a substrate for mTORC1 (mammalian target of rapamycin complex 1). In non-phosphorylated conditions, it interacts with p21 (cyclin-dependent kinase inhibitor) and enhances destabilization of p21.

General description

The gene E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1) is mapped to human chromosome 8p11-p12. It belongs to the translation repressor protein family.
Human E1F4EBP1 (eukaryotic translation initiation factor 4E binding protein 1)(GenBank Accession No. NM_004095), full length with N-terminal His tag, MW = 13.6kDa, expressed in Escherichia coli.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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