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SRP0255 Sigma

eIF4E Active human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

Synonym: CBP, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Gene Regulation, Gene Regulation and Expression
recombinant   expressed in E. coli
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 51 kDa
NCBI accession no.   NM_001968
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... EIF4E(1977)

Description

Application

Suitable substrate for MNK1 kinase.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.

Biochem/physiol Actions

EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.

General description

EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein. It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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