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SRP0256 Sigma

RB Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym: OSRC, RB1, Retinoblastoma 1, Retinoblastoma-associated protein, p105-Rb

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Cycle Proteins, Antibodies and Reagents, Cell Cycle Regulators, Cell Signaling and Neuroscience More...
recombinant   expressed in E. coli
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 43.5 kDa
NCBI accession no.   NM_000321
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... RB1(5925)

Description

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

Application

Retinoblastoma-associated protein (RB1) has been used in cell activity assay. It is also used as a kinase substrate for the kinase assay and in Western blotting.

Biochem/physiol Actions

Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma.

General description

The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2. Human recombinant RB protein (773-end) with N-terminal GST tag (GenBank Accession No. NM_000321), expressed in a E. coli expression system.

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Flow Cytometry Instruments

All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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