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SRP0275 Sigma

PDE10A Active from rat

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: Pde10a3, Phosphodiesterase 10A, Testis-specific phosphodiesterase PDE10A2, cAMP and cAMP-inhibited cGMP 3′,5′-cyclic phosphodiesterase 10A

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cyclic Nucleotide Metabolism, Cyclic Nucleotide Metabolism Enzymes, G Proteins and Cyclic Nucleotides More...
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 66 kDa
NCBI accession no.   NM_022236
shipped in   dry ice
storage temp.   −70°C
Gene Information   rat ... PDE10A(63885)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Rat PDE10A GenBank Accession No. NM_022236), amino acids 449-end with N-terminal GST tag, MW = 66 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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