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SRP0278 Sigma

PDE11A4 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym: PPNAD2, Phosphodiesterase 11A4, cAMP and cGMP cyclic nucleotide phosphodiesterase 11A

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cyclic Nucleotide Metabolism, Cyclic Nucleotide Metabolism Enzymes, G Proteins and Cyclic Nucleotides More...
recombinant   expressed in baculovirus infected insect cells
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 131 kDa
NCBI accession no.   BAB62712
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... PDE11A(50940)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will convert 1 pmole of 3′, 5′-cAMP to 5′ AMP per min at 37°C

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, 5 mM glutathione and 3 mM DTT.

Biochem/physiol Actions

Phosphodiesterases (PDEs) function as regulators of cyclic nucleotide levels. PDE11A is a dual-specificity PDE that might be involved in controlling both cGMP and cAMP levels under physiological conditions. A genome-wide study shows that the inactivation of this gene is linked with adrenocortical hyperplasia. Polymorphisms in this gene are also linked with ACTH (adrenocorticotropic hormone)-independent macronodular adrenal hyperplasia (AIMAH). PDE11A is also involved in the control of cortisol secretion. In Carney complex (CNC) patients with germline PRKAR1A mutations, polymorphisms in PDE11A might influence the development of testicular and adrenal tumors by acting as a modifier of the phenotype.

General description

PDE11A (phosphodiesterase 11A) belongs to the PDE protein family, and is expressed in three isoforms. Its mRNA has been found to be expressed in skeletal muscle, prostate, kidney, liver, pituitary, and salivary glands and testis. All PDEs contain a conserved PDE catalytic domain at the C-terminal, which also contains the PDE signature motif HDX2HX4N. This gene is localized to human chromosome 2q31–2q35.
Human PDE11A4 (GenBank Accession No. BAB62712), full length with N-terminal GST tag, MW = 131kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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