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SRP0279 Sigma

UBA6 (UBE1L2) Active human

recombinant, expressed in baculovirus infected insect cells, ≥70% (SDS-PAGE)

Synonym: MOP-4, UBE1L2, Ubiquitin-activating enzyme E1-like 2, Ubiquitin-like modifier activating enzyme 6

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Intracellular Protein Degradation, Nitric Oxide and Cell Stress, Ubiquitin and Proteases More...
recombinant   expressed in baculovirus infected insect cells
assay   ≥70% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 118 kDa
NCBI accession no.   NM_018227
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... UBA6(55236)

Description

Application

Useful in conjunction with E2 and E3 for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human UBA6 (UBE1L2) (GenBank Accession No. NM_018227), amino acids 2-end, with N-terminal DDDDK tag (FLAG), MW = 118 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 100 μg/ml FLAG peptide, 20% glycerol and 3 mM DTT.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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