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SRP0280 Sigma

UbcH13 (UBE2N) Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Synonym: UBC13 homolog, UBE2N, UbcH13, Ubiquitin-conjugating enzyme E2N

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Intracellular Protein Degradation, Nitric Oxide and Cell Stress, Ubiquitin and Proteases More...
recombinant   expressed in E. coli
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 17.6 kDa
NCBI accession no.   NM_003348
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... UBE2N(7334)

Description

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

UbcH13 (GenBank Accession No. NM_003348), amino acids 2-end, with N-terminal His-tag, MW = 17.6 kDa, expressed in an E. coli cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
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