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SRP0283 Sigma

Sphingosine kinase 1 Active human

recombinant, expressed in baculovirus, ≥80% (SDS-PAGE)

Synonym: SK1, SPHK1, SPK 1



Related Categories Active Kinases, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology
recombinant   expressed in baculovirus
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 46.9 kDa
NCBI accession no.   AF200328
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... SPHK1(8877)



Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

General description

Human Sphingosine kinase 1 (GenBank Accession No. AF200328), full length with N-terminal His tag, MW = 46.9 kDa, expressed in a Baculovirus infected Sf9 cell expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

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