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SRP0287 Sigma

Sphingosine kinase 2 (long form) Active human

recombinant, expressed in baculovirus, ≥80% (SDS-PAGE)

Synonym: SK2, SPHK2, SPK 2

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Properties

Related Categories Active Kinases, Cell Biology, Cell Signaling and Neuroscience, Kinase/Phosphatase Biology
recombinant   expressed in baculovirus
assay   ≥80% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 74 kDa
NCBI accession no.   NM_020126
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... SPHK2(56848)

Description

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit of sphingosine kinase activity is defined as the amount of enzyme required to produce 1 pmol of S1P/min

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol and 3 mM DTT.

Application

Sphingosine kinase 2 is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Sphingosine kinase 2 has been used in a study to assess neuroapoptosis in the developing mouse brain. Sphingosine kinase 2 assays were used to confirm FTY720 bioactivity in a study investigating cranial allograft incorporation.

General description

Glucose activates sphingosine kinase 2 in pancreatic β cells leading to a rise in S1P levels, which is important for glucose-stimulated insulin secretion. Human sphingosine kinase 2 (GenBank Accession No.NM_020126), is full length with an N-terminal His tag, has a molecular weight of 74 kDa, and is expressed in a Baculovirus infected Sf9 cell expression system.

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Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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