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SRP0288 Sigma

ATP Citrate Lyase Active human

recombinant, expressed in baculovirus, ≥90% (SDS-PAGE)

Synonym: ACLY, ATP-citrate synthase, ATPCL, CLATP



Related Categories Cancer Metabolism, Cancer Research, Cell Biology, Fatty Acid Metabolism in Oncogenesis, Proteins Involved in Fatty Acid Metabolism More...
recombinant   expressed in baculovirus
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 147 kDa
NCBI accession no.   NM_001096
shipped in   dry ice
storage temp.   −70°C
Gene Information   mouse ... ACLY(47)


Biochem/physiol Actions

ATP citrate lyase is an enzyme involved in fatty acid synthesis that generates cytosolic acetyl-CoA and oxaloacetate from citrate and CoA. ATP citrate lyase is often upregulated in cancer.

General description

Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST tag, MW = 147 kDa, expressed in baculovirus expression system.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme required to convert 1 pmol of ADP to ATP/min at 37°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.


Active human ATP citrate lyase is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human ATP citrate lyase has been used in a study to ascertain the nature of the catalytic phosphorylation that initiates the ACL reaction, and to identity the active site residues involved. Active human ATP citrate lyase has also been used in a study to analyze tumor metabolism to reveal mitochondrial glucose oxidation in genetically diverse human glioblastomas.

Price and Availability

All labs need water
Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis

Protocols & Articles


Fatty Acid Synthesis and Metabolism in Cancer Cells

Proliferatively active cells require fatty acids for functions such as membrane generation, protein modification, and bioenergetic requirements. These fatty acids are derived either from dietary sour...
Keywords: Apoptosis, Biofiles, Cancer, Carboxylations, Catalysis, Citric Acid Cycle, Gene expression, Glycolysis, Metabolism, Oxidations, PAGE, Phosphorylations

Peer-Reviewed Papers


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