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SRP0289 Sigma

Cathepsin B Active human

recombinant, expressed in FreeStyle 293-F cells, ≥90% (SDS-PAGE)

Synonym: APP secretase, CPSB

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Properties

Related Categories Apoptosis and Cell Cycle, Cathepsins and Granzymes, Cathepsins and Granzymes Enzymes, Cell Biology, Cell Signaling and Neuroscience More...
recombinant   expressed in FreeStyle 293-F cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 43 kDa
NCBI accession no.   NM_001908
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... CTSB(1508)

Description

General description

Human cathepsin B (GenBank Accession No. NM_001908), CD33 signal peptide(amino acids 1-16) + CathepsinB (amino acids 18-339), with C-terminal HIS tag, MW = 43 kDa, expressed in FreeStyle 293-F cells.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 200 mM imidazole.

Application

Active human cathepsin B is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin B has been used in a study to assess maturation through proenzyme activation and proteolytic processing. Active human cathepsin B has also been used in a study to prepare a catalytically active version from its precursor expressed in Escherichia coli in the form of inclusion bodies.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

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Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
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