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SRP0291 Sigma

Cathepsin L Active human

recombinant, expressed in FreeStyle 293-F cells, ≥90% (SDS-PAGE)

Synonym: CATL, Major excreted protein (MEP)

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Properties

Related Categories Apoptosis and Cell Cycle, Cathepsins and Granzymes, Cathepsins and Granzymes Enzymes, Cell Biology, Cell Signaling and Neuroscience More...
recombinant   expressed in FreeStyle 293-F cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 36 kDa
NCBI accession no.   NM_001912
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... CTSL(1514)

Description

General description

Human cathepsin L (GenBank Accession No. NM_001912), amino acids 18-333, with C-terminal HIS tag, MW = 36 kDa, expressed in FreeStyle 293-F cells.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 50 mM MES, 400 mM NaCl, pH 5.0, and 10% glycerol.

Application

Active human cathepsin L is useful for the activation of DPP1 (Cathepsin C). Active human cathepsin L has been used in a study to determine that N-acyl and N-sulfonyloxazolidine-2,4-diones are pseudo-irreversible inhibitors of serine proteases. Active human cathepsin L has also been used in a study to investigate the role of cathepsin B and L activity in the serum during the human aging process.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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