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SRP0292 Sigma

Cathepsin S Active human

recombinant, expressed in FreeStyle 293-F cells, ≥90% (SDS-PAGE)

Synonym: MGC3886

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Properties

Related Categories Apoptosis and Cell Cycle, Cathepsins and Granzymes, Cathepsins and Granzymes Enzymes, Cell Biology, Cell Signaling and Neuroscience More...
recombinant   expressed in FreeStyle 293-F cells
assay   ≥90% (SDS-PAGE)
form   aqueous solution
mol wt   mol wt 37 kDa
NCBI accession no.   NM_004079.3
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... CTSS(1520)

Description

General description

Human cathepsin S (GenBank Accession No. NM_004079.3), CD33 signal peptide(amino acids 1-16) + cathepsin S (amino acids 17-331), with C-terminal HIS tag, MW = 37 kDa, expressed in FreeStyle 293-F cells.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Unit Definition

One unit is defined as the amount of enzyme that will cleave 1 pmol of substrate per min at 37°C

Physical form

Formulated in 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 225 mM imidazole, 3 mM DTT, and 10% glycerol.

Application

Active human cathepsin S is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human cathepsin S has been used in a study to assess the pathogenesis of Alzheimer′s disease. Active human cathepsin S has also been used in a study to investigate the optimization of selectivity of Azepanone-based inhibitors.

Legal Information

FreeStyle is a trademark of Invitrogen Corp.

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Safety Information

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Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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