Stemgent^® Lentivirus hcMyc

Frequently Asked Questions

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Analysis Note

Viral concentration determined by p24 (capsid antigen) ELISA assay. Transcription factor protein expression verified by immunocytochemistry analysis of transduced human cells.

Biochem/physiol Actions

The expression of the four human transcription factors (TFs) (Oct4, Klf4, Sox2, and c-Myc) has been shown to reprogram human adult fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell. Each unique virus is provided as a VSV-G pseudotyped virus stock capable of efficiently transducing a wide range cell types.

Caution

The greatest safety risk associated with viral delivery systems stems from the potential generation of recombinant viruses that are capable of autonomous replication during the packaging process. This platform eliminates these hazards by combining a disabled viral genome with a unique manufacturing process. Also, gene functions that facilitate the enclosing of the silencing sequence in a viral capsid (e.g., Gag, Pol, Env) are distributed into multiple helper plasmids (which do not contain significant regions of homology) during packaging. This tactic further minimizes the probability of recombination events that might otherwise generate viruses capable of autonomous replication. With these safety measures in place, the Stemgent iPSC Lentivirus particles can be employed in standard Biosafety Level 2 tissue culture facilities and should be treated with the same level of caution as with any other potentially infectious agent. Also, please note that the c-Myc and Klf4 proteins are reported to demonstrate oncogenic properties.

General description

The Stemgent Lentivirus hcMyc provides an affordable method for generating iPS cells. The hc-Myc lentivirus, along with the hOct4, hKlf4, and hSox2 prepackaged lentivirus, is tested and proven to reprogram human fibroblast cells.

Other Notes

Concentration titer(s) for this product are lot specific. If you have not purchased this product, please contact Sigma′s Technical Service group for the concentration titers. If you have purchased, please consult the online Certificate of Analysis.

Legal Information

Stemgent is a registered trademark of Stemgent, Inc.