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STR1 Sigma

Enhanced Avian First Strand Synthesis Kit

Components for cDNA synthesis with enhanced AMV reverse transcriptase

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Properties

Related Categories Molecular Biology, PCR/Amplification, RT-PCR and RT-qPCR More...
usage   1 kit sufficient for 50 reactions
color   colorless
shipped in   dry ice
storage temp.   −20°C

Description

Application

Enhanced Avian First Strand Synthesis Kit utilizes a highly purified avian myeloblastosis virus reverse transcriptase (eAMV-RT) that offers superior performance in comparison to standard AMV-RT or standard Moloney murine leukemia virus reverse transcriptase (MMLV-RT). This exceptionally robust eAMV-RT has an enhanced ability to transcribe through difficult secondary structure at elevated temperatures (up to 65 °C) making it the ideal enzyme for producing high quality full-length cDNA from total RNA or poly(A)+ RNA.

Legal Information

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

Unit Definition

One unit incorporates one nanomole of TMP into TCA-precipitable material in 10 minutes using polyadenylic acid as template and oligo(dT)12-18 as primer.

Price and Availability


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Kapa Biosystems

Kit component only

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Enhanced Avian Reverse Transcriptase 1000 U SDS    

Standard component

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Deoxynucleotide mix 50 μL SDS C1595
Anchored oligo (dT)23 100 μL SDS O4387
Random nonamers 100 μL SDS R7647
Ribonuclease inhibitor 50 μL SDS R2520
PCR grade water 1.5 mL SDS W1754
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
Protocols & Articles

Articles

Reverse Transcription - PCR Technologies Guide

One approach to the analysis of gene expression is to measure the concentration of mRNA of a gene. There are several challenges to such analyses, such as the differences in halflife between different...
Keywords: Degradations, Detection methods, Gene expression, Nucleic acid hybridization, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription

Protocols

The 3'/5' Assay for Analysis of RNA Integrity Protocol

The 3’/5’ integrity assay is a potential first step in the identification of RNA degradation. The assay is particularly useful when a large number of samples are to be analyzed or when the degradatio...
Keywords: Amplification, Degradations, Nucleic acid denaturation, Polymerase chain reaction, Polymerase chain reaction - quantitative, Size-exclusion chromatography, Transcription

Related Content

PCR Selection Guide

Sigma-Aldrich offers a wide variety of PCR reagents to meet any experimental needs. Our range of polymerases is customized to meet your End-Point PCR, qPCR, or RT-PCR needs. Our products vary from ro...
Keywords: Polymerase chain reaction, Polymerase chain reaction - quantitative

RT-PCR Fundamentals

Reverse transcriptase PCR, or RT-PCR, is a variation of standard PCR technique, which involves the amplification of specific mRNA obtained from very small samples. It eliminates the need for the tedi...
Keywords: Amplification, Cloning, Diagnostic, Microbiology, Molecular biology, Polymerase chain reaction, Polymerase chain reaction - quantitative, Purification, Transcription, Whole genome amplification

Peer-Reviewed Papers
15

References

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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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