Spectrum™ Plant Total RNA Kit
sufficient for 50 purifications
DOWNLOAD MSDS (PDF)-
Popular Documents: Bulletin (PDF)
Properties
| Related Categories | Plant Biotechnology, Plant Molecular Biology, Plant Nucleic Acid Purification, RNA Purification |
| usage | sufficient for 50 purifications |
| storage temp. | 20-25°C |
Description
Features and Benefits
• Yields up to 60 μg of pure concentrated RNA per prep
• Efficient protocol allows for RNA purification in 30 min or less
• Specially designed for research with difficult plant tissues
General description
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
Legal Information
Spectrum is a trademark of Sigma-Aldrich Co. LLC
Price and Availability
Customers Also Viewed
Amplification Grade
sufficient for 10 preparations
sufficient for 70 preparations
BioUltra, for molecular biology
Related Papers
Technical Service:
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Bulk Ordering & Pricing:
Need larger quantities for your development, manufacturing or research applications?
