|Related Categories||Plant Biotechnology, Plant Molecular Biology, Plant Nucleic Acid Purification, RNA Purification|
|usage||sufficient for 50 purifications|
• Yields up to 60 μg of pure concentrated RNA per prep
• Efficient protocol allows for RNA purification in 30 min or less
• Specially designed for research with difficult plant tissues
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
Spectrum is a trademark of Sigma-Aldrich Co. LLC
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Certificate of Analysis
|Symbol||GHS05, GHS06, GHS08, GHS09|
|Hazard statements||H301 + H331-H310-H315-H317-H318-H335-H373-H410|
|Precautionary statements||P261-P273-P280-P301 + P310-P302 + P350-P305 + P351 + P338|
|Hazard Codes (Europe)||T,N|
|Risk Statements (Europe)||23/24/25-37/38-41-43-48/22-50/53|
|Safety Statements (Europe)||26-36/37/39-45-61|
|RIDADR||UN 2966 6.1/PG 2|
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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