|Related Categories||Molecular Biology, Plant Biotechnology, Plant Molecular Biology, Plant Nucleic Acid Purification, RNA Purification More...|
|usage||sufficient for 50 purifications|
• Yields up to 60 μg of pure concentrated RNA per prep
• Efficient protocol allows for RNA purification in 30 min or less
• Specially designed for research with difficult plant tissues
The Spectrum Plant Total RNA Kit utilizes a lysis and binding chemistry and a convenient column-based ′bind-wash-and-elute′ format to purify up to 100 μg of total RNA from 100 mg of tissue in about 30 minutes. Typical yields range from 20–60 μg.
After grinding tissue to a fine powder in liquid nitrogen, cells are lysed and cellular debris is physically and chemically separated from endogenous RNA. RNA is then bound to a column supported silica substrate and several wash steps remove remaining contaminants.
Total RNA is eluted from the column and used in typical applications, such as Northern Blots, and RT- and qRT-PCR.
Spectrum is a trademark of Sigma-Aldrich Co. LLC
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Certificate of Analysis
|Symbol||GHS05, GHS06, GHS08, GHS09|
|Hazard statements||H301 + H331-H310-H315-H317-H318-H335-H373-H410|
|Precautionary statements||P261-P273-P280-P301 + P310-P302 + P350-P305 + P351 + P338|
|Hazard Codes (Europe)||T,N|
|Risk Statements (Europe)||23/24/25-37/38-41-43-48/22-50/53|
|Safety Statements (Europe)||26-36/37/39-45-61|
|RIDADR||UN 2966 6.1/PG 2|
A combined functional and structural genomics approach identified an EST-SSR marker with complete linkage to the Ligon lintless-2 genetic locus in cotton (Gossypium hirsutum L.). Hinchliffe DJ, Turley RB, Naoumkina M, et al. BMC Genomics 12, 445, (2011)
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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