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T0319 Sigma

Trizma® Pre-set crystals

BioPerformance Certified, pH 7.4, average Mw 151.6

Synonym: Tris crystals



Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffers A to Z,
grade   BioPerformance Certified
mol wt   average Mw 151.6
impurities   DNase, RNase, Protease, NICKase, none detected
  endotoxin, tested
  total aerobic microbial count, tested
  ≤0.05 A290
  ≤0.5% water (Karl Fischer)
  ≤5 ppm Heavy metals (as Pb)
pH   7.12 (37 °C, 0.1 M)(lit.)
  7.40 (25 °C, 0.1 M)(lit.)
  7.97 (5 °C, 0.1 M)(lit.)
useful pH range   7.0 - 9.0
suitability   cell culture tested
Featured Industry   Diagnostic Assay Manufacturing



The product is cell culture tested. It is also tested for low endotoxin levels and analyzed for the absence of nucleases and proteases.

Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

Other Notes

This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.

Certain pH electrodes do not give accurate results with Tris buffers.


In convenient foil pouches

Preparation Note

Trizma solutions can be autoclaved.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

Interactive Buffer Table
Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Protocols & Articles

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