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T0438 Sigma

Monoclonal Anti-Transforming Growth Factor-β1 antibody produced in mouse

clone 9016.2, purified immunoglobulin, lyophilized powder

Synonym: Anti-TGF-β1

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   rat, mouse, human
application(s)   capture ELISA: suitable
  immunohistochemistry: suitable
  neutralization: suitable
  western blot: suitable
clone   9016.2, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG1
storage temp.   −20°C
Gene Information   human ... TGFB1(7040)
mouse ... Tgfb1(21803)
rat ... Tgfb1(59086)
biological source   mouse
conjugate   unconjugated
Featured Industry   Research Pathology

Description

Immunogen

purified, CHO cell-derived, recombinant TGF-β1 and latent TGF-β1.

General description

Transforming growth factor-β (TGF-β) belongs to the TGF-β superfamily of secreted proteins. TGF-β 1 exists as three isoforms TGF-β 1, TGF-β 2 and TGF-β 3. The TGF-β family is involved in a variety of signaling pathways that involve serine/threonine protein kinases. TGF-β acts through binding to three transmembrane receptors in mammals, type I, type II and type III receptors. The important pathways regulated by TGF-β are the SMAD, BMP, MAPK and PI3K/Akt pathways. TGF-β 1 is involved in cell and tissue differentiation, proliferation, inflammation, migration of monocytes, neutrophils and chemotaxis. Deregulation of TGF-β signaling has been implicated in many inflammation and autoimmune diseases. Monoclonal Anti-Transforming Growth Factor-β 1 antibody specifically reacts with human, mouse and rat TGF-β 1. The antibody shows less than 2% cross reactivity with TGF-β3 and TGF-β5 in ELISA.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Specificity

By capture ELISA, <2% cross-reactivity is observed with TGF-β3 and TGF-β5.

Preparation Note

Purified using protein G

Application

Anti-TGF-β I antibody may be used for immunoblotting at a working concentration of 1-2 μg/ml. The antibody may be used for ELISA as a capture antibody at a concentration of 1 μg/ml. A working antibody concentration of 8-25 μg/ml may be used for immunohistochemistry. For immunohistochemistry of corpus cavernosal biopsy tissue sections, a dilution of 1:50 was used.

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