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T0440 Sigma

3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA

peroxidase substrate

Synonym: TMB



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TMB is a substrate for horseradish peroxidase. Develops a soluble blue reaction product that may be read at 370 or 655 nm. For endpoint assays the reaction may be stopped with acid, forming a yellow reaction product that may be read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications.


Stable at least one year at 2-8 °C.

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