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T0665 Sigma

holo-Transferrin human

powder, BioReagent, suitable for cell culture, ≥97%

Synonym: Siderophilin, iron-saturated

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Properties

Related Categories Albumins and Transport Proteins, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines, Growth Factors and Hormones,
grade   Cell Culture
description   Iron-saturated
product line   BioReagent
assay   ≥97%
form   powder
impurities   HIV and HBsAg, none detected
  endotoxin, tested
solubility   H2O: 50 mg/mL
suitability   suitable for cell culture
storage temp.   2-8°C
Gene Information   human ... TF(7018)

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Analysis Note

Iron content by ICP reported on a lot-specific basis.

Purity by agarose gel electrophoresis.

Packaging

1 g in poly bottle

50, 100, 500 mg in poly bottle

Preparation Note

Heat treated at 60 °C (minimum) for a minimum of 10 hours.

Application

Transferrin is the primary iron binding and cell delivery molecule present in serum. Serum-free cell culture systems require a delivery format for iron. Transferrin is the preferential delivery form of iron because cells process transferrin bound iron in a physiologically appropriate way through transferrin receptors on the cell surface. Human holo-transferrin is a high affinity transferrin that can be used with a wide range of cells types. Human holo-transferrin is loaded with iron prior to use and can be added directly to iron poor or iron-free cell culture media.

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the carbohydrate content of human transferrin?
We have found the information on transferrin in a reference book: The Plasma Proteins, F.W. Putnam, ed, volume 2, chapter 4, Table II. According to the table, Transferrin is 6 percent carbohydrate, citing G.A. Jamieson, J. Biol. Chem. 240(7), 2914-2920 (1965). The table also gives information on the percentage of each type of monosaccharide that is present.
  What kind of solution is the A5955 - Antibiotic Antimycotic Solution (100×), Stabilized product suspended in?
A5955 - Antibiotic Antimycotic Solution (100×), Stabilized is supsended in a sodium citrate buffer solution. Source: Israel production group
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Protocols & Articles

Articles

HPLC Analysis of Plasma Proteins on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Plasma Proteins on BIOshell™ A400 Protein C4
Keywords: Chromatography, Clinical, High performance liquid chromatography

HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Immobilized Metal Ion Affinity Chromatography on TSKgel® Chelate-5PW
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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