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Transgenesis Techniques: Principles and Protocols, 2nd ed.

  •  ISBN-10 0-89603-696-0

  •  ISBN-13 978-0-89603-696-3

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Related Categories Books and Software, Genetics Books, Labware, Molecular Biology Books
publication info   A.R. Clarke, Humana Press, 2002, 368 pp., hard cover
mfr. no.   Humana

Description

General description

This update presents techniques for generation and analysis of transgenic animals, with emphasis on manipulation of the mammalian genome. It concentrates on creation and maintenance of genetically modified murine strains, providing access to the germline by conventional pronuclear injection, and by retroviral and adenoviral infection. Emphasis is given to the generation, maintenance, and manipulation of embryonic stem cell lineages, with protocols for constitutive and conditional gene targeting. Chapters include techniques for cryopreservation of male and female germlines and for generation of transgenic sheep by nuclear transfer.

Table of Contents

Part I. Topical Reviews in Transgenesis. Biomedical and Agricultural Applications of Animal Transgenesis,
Part II. Transgenesis in Invertebrate and Lower Vertebrate Species. Gene Transfer in Drosophila,
Part III. Transgenesis in the Mouse: Oocyte Injection. Oocyte Injection in the Mouse,
Part IV. Alternative Routes to the Germline. Adenoviral Infection,
Part V. Transgenesis in the Mouse: The ES Cell Route. In Vitro Isolation of Murine Embryonic Stem Cells,
Production of Chimeras Derived from Murine Embryonic Stem Cells,
Gene Targeting Strategies,
Cre/loxP Recombination System and Gene Targeting, R
Part VI. Cryopreservation of Mouse Lines. Cryopreservation of Transgenic Mouse Lines,
Ovarian Tissue Transplantation and Cryopreservation: Application to Maintenance and Recovery of Transgenic and Inbred Mouse Lines,
Part VII. Transgenesis in the Rat. Transgenesis in the Rat,
Part VIII. Transgenesis in Domestic Species. Generation of Transgenic Livestock by Pronuclear Injection,
Transgenic Sheep from Cultured Cells,
Part IX. Characterization and Analysis of Transgenic Strains. Analysis of Transgenic Mice,
Index

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