• USA Home
  • T0813 - Anti-Tumor Necrosis Factor-α antibody produced in goat

EMAIL THIS PAGE TO A FRIEND
T0813 Sigma

Anti-Tumor Necrosis Factor-α antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-TNF-α

Description

Immunogen

recombinant human TNF-α.

General description

Tumor Necrosis Factor-α (TNF-α) is a cytokine produced by macrophages in response to stimulus by LPS. It plays a major role in mediating inflammation, tissue injury, pathogenic shock, innate immunity, apoptosis and autoimmunity. The physiological effects of TNF-α are mediated by receptors of TNFR super family. The TNF/TNFR signaling axis plays a critical role in mediating either survival or apoptosis depending on the class of adaptor proteins recruited in the cell. Association of receptors with a death domain causes rapid activation of caspases and results in cell death. TNF/TNFR also results in the activation of downstream pathways involving MAPK, p38, JNK and NF-κB that play a major role in innate immunity, acute inflammatory responses and homeostasis. The TNF/TNFR axis is also important in the pathology of various autoimmune and inflammatory disorders including a variety of malignancies and Alzheimer′s disease. Anti-Tumor Necrosis Factor-α antibody is specific for human TNF-α. The antibody shows less than 10% cross reactivity with recombinant rabbit TNF-α and less than 5% cross reactivity with recombinant mouse TNF-α.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline containing 5% trehalose.

Specificity

The antibody neutralizes the biological activity of recombinant human TNF-α. The antibody shows <10% cross-reactivity with recombinant rat TNF-α and <5% cross-reactivity with recombinant mouse TNF-α.

Preparation Note

The antibody is purified using TNF-α affinity chromatography.

Application

Anti-Tumor Necrosis Factor-α antibody may be used for immunoblotting at a working antibody concentration of 0.1 μg/ml. For immunohistochemistry a concentration range of 5-15 μg/ml is recommended. The antibody may be used for neutralization assay; the ND50 is 0.02-0.12 μg/ml.

Price and Availability


Detect Your Unseen Protein

All labs need water

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?