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T0910 Sigma

Thioredoxin from Escherichia coli

recombinant, expressed in E. coli, essentially salt-free, lyophilized powder, ≥3 units/mg protein

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Properties

Related Categories Cell Biology, Cell Signaling and Neuroscience, Cell Stress, Nitric Oxide and Cell Stress, Oxidative Stress Proteins and Reagents More...
recombinant   expressed in E. coli
assay   ≥90% (SDS-PAGE)
form   essentially salt-free, lyophilized powder
mol wt   mol wt 11.7 kDa
storage temp.   −20°C
Gene Information   Escherichia coli K12 ... trxA(948289)

Description

Biochem/physiol Actions

Mediates the reduction of disulfide bonds in proteins.

Thioredoxin from Escherichia coli catalyzes REDOX reactions and has a role in various biological processes. It modulates the structure and activity of many proteins. The enzyme thioredoxin reductase catalyzes the reduction of thioredoxin. Thioredoxin also has a role in oxidative stress response.

Other Notes

GenBank® Accession Number M26133.

Packaging

1 mg in glass bottle

Unit Definition

One unit will cause a ΔA650 of 1.0 in 1 min at 25 °C in the insulin reduction assay.

Application

Thioredoxin from Escherichia coli has been used in thioredoxin reductase assay, peroxidase-coupled thioredoxin system assay and peroxiredoxin assay.

General description

Thioredoxin from Escherichia coli is a monomeric, globular protein. It has a central β-sheet surrounded by α-helices.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services

Price and Availability


All labs need water
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins (2) on BIOshell™ A400 Protein C4
Keywords: Chromatography, High performance liquid chromatography, Reversed-phase chromatography

Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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