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T0938 Sigma

Anti-Tumor Necrosis Factor-α antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-TNF-α

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors,
species reactivity   mouse
application(s)   capture ELISA: suitable
  flow cytometry: 5-25 μg/mL
  indirect immunofluorescence: 15 μg/mL using mouse cells and tissues
  neutralization: suitable
  western blot: 0.1-0.2 μg/mL
clone   polyclonal
antibody form   affinity isolated antibody
form   lyophilized powder
storage temp.   −20°C
Gene Information   mouse ... Tnf(21926)
biological source   goat
conjugate   unconjugated

Description

Immunogen

recombinant mouse TNF-α

General description

Tumor Necrosis Factor-α (TNF-α) is a protein produced by lipopolysaccharide-stimulated macrophages and causes tumor necrosis. It also facilitates many functions like stimulate growth of human fibroblasts and other cell lines, activate polymorphonuclear neutrophils and osteoclasts and collagenase production. Anti-Tumor Necrosis Factor-α antibody can be used for neutralizing the biological activity of recombinant mouse TNF-α. It can also be used in indirect immunofluorescence. Goat anti-Tumor Necrosis Factor-α antibody reacts specifically with recombinant mouse TNF-α.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Specificity

The antibody neutralizes the biological activity of recombinant mouse TNF-α.

Preparation Note

Purified using mouse TNF-α affinity chromatography.

Application

Anti-Tumor Necrosis Factor-α antibody can be used in ELISA capture and flow cytometry. It can also be used in immunoblotting.

Price and Availability


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