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T1001 Sigma

Thyroglobulin from bovine thyroid

powder, ≥90% (agarose gel electrophoresis)

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Properties

Related Categories Antibodies, Antibody Purification and Characterization, Application Index, Biochemicals and Reagents, Carrier Proteins,
assay   ≥90% (agarose gel electrophoresis)
form   powder
mol wt   mol wt ~670 kDa
composition   Iodine, ≥0.20%
nitrogen analysis   13.0-16.0% total
ash   ≤4%
solubility   H2O: 20 mg/mL
storage temp.   −20°C
Gene Information   cow ... TG(280706)

Description

Application

Bovine thyroglobulin was shown to stimulate proliferation in rat thyroid follicular cells as well as in primary porcine thyroid epithelial cells. This effect was mediated through PI3K signaling.

Often used as a carrier protein for the production of antibodies. Its advantage is the large number of tyrosine residues that can be used for conjugation using the diazo reaction.

Biochem/physiol Actions

Bovine thyroglobulin is a large, tetrameric glycoprotein produced in the thyroid gland as a precursor to thyroid hormones. Its large size and absence from plasma under normal conditions makes it very immunogenic and reduces the chance that antibodies to this carrier protein will cross-react with plasma proteins in immunoassays. Haptens may be conjugated to thyroglobulin via accessible secondary amines.

Iodine-containing protein that is stored in the thyroid gland. Converted into circulating thyroxines when the thyroid is stimulated by thyroid-stimulating hormone (TSH) from the pituitary. Because its expression is unique to the thyroid, it is a sensitive indicator of recurrence or metastasis of thyroid cancer that has been treated by thyroid ablation.

Packaging

1, 5 g in poly bottle

100, 500 mg in poly bottle

Price and Availability


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Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

A Validated Amino Acid Analysis Assay for Accurate Quantification of Stable Isotope Labeled Protein Reference Materials: SIL Thyroglobulin Case Study

With the emergence of mass spectrometry for the clinical measurement of proteins in biological matrices, the development of biological reference materials will continue to grow in importance. Certifi...
Kevin Ray1, Pegah R. Jalili1, Mark Angeles1, James J. Walters1, Isil Dilek2, and Uma Sreenivasan2

1 - MilliporeSigma, St. Louis, MO
2 - MilliporeSigma, Round Rock, TX
Keywords: Amino acid analysis, Clinical, Fractionation, High performance liquid chromatography, Mass spectrometry, PAGE, Protein extraction

Characterization of Clinically-Relevant Stable Isotope Labeled Recombinant Proteins For Use As Internal Standards in Quantitative MS Workflows

Methods for quantification of clinical protein biomarkers by mass spectrometry demand a high level of analytical quality. Procedures must be developed to minimize and control experimental variations ...
Kevin Ray, Pegah Jalili, James Walters, David Rhee
Sigma-Aldrich, St. Louis, MO
Keywords: Clinical, Fractionation, Mass spectrometry, Protein extraction

HPLC Analysis of Four Proteins on Zenix® SEC-300, Comparison of Column Internal Diameter

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Four Proteins on Zenix® SEC-300, Comparison of Column Internal Diameter
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size: 100Å vs. 300Å

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards by Size Exclusion on Zenix® as Affected by Pore Size: 100Å vs. 300Å
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate
Keywords: Chromatography, Diffusion, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Arginine

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Arginine
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Potassium Chloride

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Potassium Chloride
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Sodium Perchlorate

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 by Size Exclusion As Affected by Sodium Perchlorate
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 in the Presence of a Denaturant

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 in the Presence of a Denaturant
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Protein Standards on Zenix® SEC-300 using Different Buffer Systems
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® Columns, Effect of Pore Size
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (15 cm column)
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins by Size Exclusion on Zenix® and SRT® SEC-300, Comparison of Particle Size and Column I.D.

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins by Size Exclusion on Zenix® and SRT® SEC-300, Comparison of Particle Size and Column I.D.
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-1000, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-1000, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-150, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-150, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm particles
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-300, 30 cm x 7.8 mm I.D., 5 μm
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on SRT® SEC-500, 30 cm x 7.8 mm I.D.

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on SRT® SEC-500, 30 cm x 7.8 mm I.D.
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 4.6 mm I.D., 3 μm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 4.6 mm I.D., 3 μm
Keywords: Chromatography, High performance liquid chromatography, Separation, Size-exclusion chromatography

HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 7.8 mm I.D., 3 μm

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Proteins on Zenix® SEC-300, 30 cm x 7.8 mm I.D., 3 μm
Keywords: Chromatography, High performance liquid chromatography, Separation, Size-exclusion chromatography

HPLC Analysis of a Protein Mixture on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of a Protein Mixture on Zenix® SEC-150 versus Zenix® SEC-300, Effect of Pore Size on Resolution
Keywords: Chromatography, Clinical, High performance liquid chromatography, Size-exclusion chromatography

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size

From our library of Articles, Sigma-Aldrich presents Protein Molecular Weight Calibration Curve on Zenix® Size Exclusion Columns, Effect of Pore Size
Keywords: Chromatography, High performance liquid chromatography, Size-exclusion chromatography

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Peer-Reviewed Papers
15

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