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T1032 Sigma

TNM-FH insect medium

With L-glutamine, without sodium bicarbonate, powder, suitable for insect cell culture

Synonym: Graces Modified Medium

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Properties

Related Categories Cell Culture, Classic Media and Salts, Insect Cell Culture Media, TNM-FH Insect Medium
Quality Level   GMP
form   powder
concentration   50.6 g/L
suitability   suitable for culture of other lepidopteran cell lines (Originally developed for the culture of Trichoplusia ni cells)
  suitable for insect cell culture
storage temp.   2-8°C

Description

Application

A number of modifications of Grace′s medium were developed in several laboratories in the late 1960′s and 1970′s. Many of these modifications were developed by Dr. W.F. Hink for the growth of cells derived from the cabbage looper, Tricoplusia ni. This formula represents the modification known as TNM-FH. The medium when properly supplemented has been found to support the growth of cells derived from a variety of lepidopteran species.

TNM-FH insect medium is used for the following applications:
• Used for the purification of recombinant proteins
• Used as a medium for the growth and maintenance of cells (cells and virus) along with other components
• TNM-FH medium is modified for culturing cell lines (embryonic cells)
• Used for production and purification of recombinant hormone-sensitive lipase (HSL) and adipocyte lipid-binding protein (ALBP).
• Used in overexpression and purification of G protein-coupled receptor kinases (GRKs) Subtypes. Spodoptera frugiperda cell line Sf9 cells were grown in complete TNM-FH and were infected at high multiplicity of infection. Recombinant protein was harvested from the infected Sf9 cells.

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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