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T1165 Sigma

Tris-Tricine-SDS Buffer 10× Concentrate

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer Convenience Packaging, Buffer Solutions, Buffers,
sterility   0.2 μm filtered
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   2-8°C

Description

Analysis Note

Tested for use as a cathode buffer for Tris-Tricine gel electrophoresis to separate low molecular weight proteins.

Application

Tris-Tricine-SDS (TTS) running buffer is the cathode (upper reservoir) buffer for SDS−polyacrylamide gel electrophoresis of proteins using the Schagger and von Jagow method. The Schagger and von Jagow method is designed for the separation of small molecular weight proteins. It differs from the Laemmli method in that the glycine is replaced with tricine and the gel contains 1 M Tris-HCl, pH 8.45 instead of 0.375 M Tris-HCl, pH 8.9. Dilution of the 10X TTS buffer produces a 1X cathode buffer containing 100 mM Tris, 100 mM tricine and 0.1% SDS. Recommended running conditions is 125 volts.

Other Notes

1M Tris, 1M Tricine, 1% (w/v) SDS, pH approx. 8.2.

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Detect Your Unseen Protein

Interactive Buffer Table
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

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